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Home >> Antibodies >> CCL2 Antibody / MCP1

CCL2 Antibody / MCP1 (F49800)

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  Catalog No Formulation Size Price (USD)  
Image F49800-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49800-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Confocal immunofluorescent analysis of CCL2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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Confocal immunofluorescent analysis of CCL2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human brain tissue with CCL2 antibody
Immunofluorescence analysis of CCL2 antibody with HeLa cells. Primary Ab was followed by FITC-conjugated goat anti-rabbit lgG. FITC emits green fluorescence. Red counterstaining is PI.
Western blot analysis of CCL2 antibody and HeLa lysate. Predicted molecular weight ~11/9kDa (precursor/mature).
Flow cytometric analysis of HeLa cells using CCL2 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Primate
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P13500
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This CCL2 antibody is available for research use only.
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Description

Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. CCL2 is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Application Notes

Titration of the CCL2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 62-89 from the human protein was used as the immunogen for this CCL2 antibody.

Storage

Aliquot the CCL2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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