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Home >> Antibodies >> Cathepsin D Antibody / CTSD

Cathepsin D Antibody / CTSD (F54746)

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  Catalog No Formulation Size Price (USD)  
Image F54746-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54746-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human 1) A431, 2) MCF7, 3) K562 and 4) PANC-1 cell lysate with Cathepsin D antibody. Expected molecular weight: 43-46 kDa, 28 kDa (heavy chain), 15 kDa (light chain).
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Western blot testing of human 1) A431, 2) MCF7, 3) K562 and 4) PANC-1 cell lysate with Cathepsin D antibody. Expected molecular weight: 43-46 kDa, 28 kDa (heavy chain), 15 kDa (light chain).
IHC testing of FFPE human breast carcinoma tissue with Cathepsin D antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with Cathepsin D antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with Cathepsin D antibody (green) and DAPI nuclear stain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P07339
Localization Cytoplasmic
Applications Western blot : 1:500-1:2000
Immunofluorescence : 1:25
Immunohistochemistry (FFPE) : 1:25
Limitations This Cathepsin D antibody is available for research use only.
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Description

Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Cathepsin D antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1-412 from the human protein was used as the immunogen for the Cathepsin D antibody.

Storage

Aliquot the Cathepsin D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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