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Home >> Antibodies >> Caspase-9 Antibody

Caspase-9 Antibody (R30673)

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  Catalog No Formulation Size Price (USD)  
Image R30673 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of Caspase-9 antibody and Lane 1: SMMC-7721; 2: MCF-7; 3: CEM; 4: Jurkat; 5: Raji; 6: HeLa
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Western blot testing of Caspase-9 antibody and Lane 1: SMMC-7721; 2: MCF-7; 3: CEM; 4: Jurkat; 5: Raji; 6: HeLa
IHC-P: Caspase-9 antibody testing of human tonsil tissue
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P55211
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Caspase-9 antibody is available for research use only.
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Description

Caspase-9, also called APAF3, is an initiator caspase, encoded by the CASP9 gene. The gene is mapped to chromosome 1p36.3-p36.1 by FISH. CASP9 is identified as a member of the caspase family that participates in CASP3 activation in vitro. And it also regarded as the most upstream member of the apoptotic protease cascade that is triggered by Cytochrome C and dATP. The crystal structure of CASP9 is complex with the BIR3 in an inhibitory domain of XIAP at 2.4-angstrom resolution and the gene contains 9 exons and spans approximately 35 kb of genomic DNA. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of Cytochrome C and dATP, an event that leads to CASP9 activation. Activity increases dramatically upon association with the apoptosome complex. And the majority of CASP9 knockout mice died perinatally with a markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Caspase-9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 183-198 (SNIDCEKLRRRFSSLH-human) were used as the immunogen for this Caspase-9 antibody. This sequence is on the large subunit.

Storage

After reconstitution, the Caspase-9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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