Caldesmon Antibody / CALD1 / CAD (R32405)

Immunofluorescent staining of FFPE human SiHa cells with Caldesmon antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC testing of FFPE human intestine with Caldesmon antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.

IHC testing of FFPE mouse intestine with Caldesmon antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.

IHC testing of FFPE rat intestine with Caldesmon antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.

Western blot testing of 1) human U-2 OS, 2) human A549, 3) human HepG2, 4) human HeLa, 5) human Caco-2 and 6) mouse NIH 3T3 cell lysate with Caldesmon antibody. Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).

Flow cytometry testing of human U-87 MG cells with Caldesmon antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caldesmon antibody.