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Home >> Antibodies >> Cadherin 16 Antibody / CDH16

Cadherin 16 Antibody / CDH16 [clone CDH16/2449] (V4432)

  Catalog No Formulation Size Price (USD)  
Image V4432-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4432-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4432SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human kidney tissue with Cadherin 16 antibody (clone CDH16/2449). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cadherin 16 antibody (clone CDH16/2449) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CDH16/2449
Purity Protein A/G affinity
UniProt O75309
Localization Cell Surface, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This Cadherin 16 antibody is available for research use only.
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Description

This mAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Application Notes

Optimal dilution of the Cadherin 16 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 300-600) from the human protein was used as the immunogen for the Cadherin 16 antibody.

Storage

Aliquot the Cadherin 16 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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