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Home >> Antibodies >> BRAF V600E Antibody

BRAF V600E Antibody (F54150)

  Catalog No Formulation Size Price (USD)  
Image F54150-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F54150-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot testing of 1) BRAF-600V, 2) BRAF-V600E, 3) Raji, 4) HeLa, 5) A549, 6) T47D and 7) MDA-MB-453 lysate with BRAF V600E antibody at 1:2000. Predicted molecular weight ~85-95 kDa.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Chicken, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P15056
Applications Western Blot : 1:1000-1:2000
Limitations This BRAF V600E antibody is available for research use only.
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Description

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Application Notes

The stated application concentrations are suggested starting points. Titration of the BRAF V600E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 576-606 from human BRAF was used as the immunogen for the BRAF V600E antibody.

Storage

Aliquot the BRAF V600E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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