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Home >> Antibodies >> Bid Antibody

Bid Antibody (F42703)

  Catalog No Formulation Size Price (USD)  
Image F42703-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F42703-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Bid antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma.
Western blot analysis of Bid antibody and mouse spleen tissue lysate. Predicted molecular weight ~22 kDa.
Bid antibody flow analysis of MCF-7 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P70444
Localization Cytoplasmic
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This Bid antibody is available for research use only.
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Description

Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.

Application Notes

Titration of the Bid antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 39-68 from the mouse protein was used as the immunogen for this Bid antibody.

Storage

Aliquot the Bid antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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