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Home >> Antibodies >> BHC80 Antibody / PHF21A

BHC80 Antibody / PHF21A (RQ5390)

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  Catalog No Formulation Size Price (USD)  
Image RQ5390 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human sarcoma with BHC80 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human sarcoma with BHC80 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with BHC80 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human esophagus squama with BHC80 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human esophagus squama with BHC80 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of 1) human A549, 2) rat brain and 3) mouse brain lysate with BHC80 antibody. Expected molecular weight 83-94 kDa.
Flow cytometry testing of human HeLa cells with BHC80 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BHC80 antibody.
IHC staining of FFPE human ovarian cancer with BHC80 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta with BHC80 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q96BD5
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This BHC80 antibody is available for research use only.
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Description

PHD finger protein 21A is a protein that in humans is encoded by the PHF21A gene. The PHF21A gene encodes BHC80, a component of a BRAF35/histone deacetylase complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS).

Application Notes

Optimal dilution of the BHC80 antibody should be determined by the researcher.

Immunogen

Amino acids M1-Q97 from the human protein were used as the immunogen for the BHC80 antibody.

Storage

Store the BHC80 antibody at -20oC.

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