ASS1 Antibody / Argininosuccinate synthetase 1 [clone 7I9] (RQ6230)

IHC staining of FFPE human breast cancer with ASS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate cancer with ASS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon cancer with ASS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer with ASS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat liver with ASS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human MCF7 cells with ASS1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human HeLa, 2) human HepG2, 3) human HEK293, 4) human ThP-1, 5) human T-47D, 6) monkey kidney, 7) rat liver, 8) rat kidney, 9) mouse liver and 10) mouse kidney lysate with ASS1 antibody. Predicted molecular weight ~46 kDa.

Flow cytometry testing of human SiHa cells with ASS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ASS1 antibody.