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Home >> Antibodies >> Aldolase Antibody

Aldolase Antibody (F48149)

  Catalog No Formulation Size Price (USD)  
Image F48149-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48149-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from 1) 293, 2) RD, 3) mouse NIH3T3 and 4) rat L6 cell line using Aldolase antibody at 1:1000. Predicted molecular weight ~40 kDa.
IHC analysis of FFPE human lung carcinoma tissue stained with Aldolase antibody
Confocal immunofluorescent analysis of Aldolase antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Predicted Reactivity Rabbit
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P04075
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This Aldolase antibody is available for research use only.
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Description

Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.

Application Notes

Titration of the Aldolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 66-95 from the human protein was used as the immunogen for this Aldolase antibody.

Storage

Aliquot the Aldolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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