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Home >> Antibodies >> ALAS1 Antibody

ALAS1 Antibody (RQ5867)

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  Catalog No Formulation Size Price (USD)  
Image RQ5867 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human renal cancer with ALAS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human renal cancer with ALAS1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with ALAS1 antibody (left) and ALAS1 antibody + DAPI nuclear stain (right). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) SW620, 2) HepG2, 3) Raji, 4) rat heart and 5) mouse heart lysate with ALAS1 antibody. Predicted molecular weight ~71 kDa.
Flow cytometry testing of human 293T cells with ALAS1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ALAS1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P13196
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ALAS1 antibody is available for research use only.
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Description

This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Notes

Optimal dilution of the ALAS1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-Q244) was used as the immunogen for the ALAS1 antibody.

Storage

After reconstitution, the ALAS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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