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Home >> Antibodies >> ADA Antibody

ADA Antibody (F41596)

  Catalog No Formulation Size Price (USD)  
Image F41596-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F41596-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human duodenum tissue with ADA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
ADA antibody flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot testing of human 1) MOLT-4, 2) Jurkat, 3) HeLa, 4) HL-60 and 5) DU145 cell lysate with ADA antibody. Expected molecular weight ~41 kDa.
ADA antibody western blot analysis in HL-60 lysate. Expected molecular weight ~41 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P00813
Localization Cytoplasm, cell membrane
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Immunohistochemistry (FFPE) : 1:100-1:500
Limitations This ADA antibody is available for research use only.
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Description

Adenosine deaminase (ADA) catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. [UniProt]

Application Notes

Titration of the ADA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 287-314 from the human protein was used as the immunogen for this ADA antibody.

Storage

Aliquot the ADA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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