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Home >> Antibodies >> ACAT2 Antibody / Acetyl-CoA acetyltransferase, cytosolic

ACAT2 Antibody / Acetyl-CoA acetyltransferase, cytosolic (RQ7052)

  Catalog No Formulation Size Price (USD)  
Image RQ7052 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human HepG2 cells with ACAT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACAT2 antibody.
Western blot testing of 1) human HepG2, 2) human HeLa, 3) human Jurkat, 4) human K562 and 5) mouse liver tissue lysate with ACAT2 antibody. Predicted molecular weight: ~41 kDa (isoform 1) and ~45 kDa (isoform 2).
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9BWD1
Applications Western blot : 0.5-1 ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ACAT2 antibody is available for research use only.
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Description

Acetyl-CoA acetyltransferase, cytosolic, also known as cytosolic acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT2 (acetyl-Coenzyme A acetyltransferase 2) gene. The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the ACAT2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q31-E397) was used as the immunogen for the ACAT2 antibody.

Storage

After reconstitution, the ACAT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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