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H3K4me3 Antibody / Trimethyl Histone H3 Lysine 4 (clone RM137)

Catalog No : R20202
Applications : WB, DB, ELISA
Reactivity : All Species
Format : Purified
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Western blot of recombinant histone H3.3 (1) and acid extracts of human HeLa cells (2) using the recombinant H3K4me3 antibody at 0.5 ug/ml showed a band of Histone H3 trimethylated at Lysine 4 (K4me3) in HeLa cells.
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H3K4me3 Antibody / Trimethyl-Histone H3 (Lys4) (clone RM340)

Catalog No : R20363
Applications : ChIP, ELISA
Reactivity : Human
Format : Purified
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SNAP-ChIP (TM) / qPCR using anti-H3K4me3 antibody. Antibody (3 ug at 17 ug/ml) was tested in native ChIP with 3 ug HEK-293 chromatin (~1x10e6 cells). Specificity (left Y-axis; all bars mean ± SEM from six independent ChIP experiments; note less than 15% cross-reactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.
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